Our lab is interested in understanding the cellular and network mechanisms involved in coordination of hippocampal information processing. In particular, we explore how hippocampal inhibitory interneurons integrate synaptic inputs and produce spikes, how the firing rate and timing of different subtypes of interneurons are regulated via the activation of specific excitatory and inhibitory pathways, and how these processes are altered in neurological and neurodegenerative disorders, including epilepsy and Alzheimer's disease.
To address these questions, we use a combination of electrophysiology (field potential, patch clamp, single unit, and sharp recordings) in vitro and in vivo, two-photon microscopy (dendritic Ca2+ imaging and neurotransmitter uncaging), optogenetics, pharmacogenetics, molecular biology, immunohistochemistry, neuroanatomy and behavioural approaches in transgenic mouse models.
Our laboratory is funded by the Natural Sciences and Engineering Research Council of Canada, Canadian Institutes of Health Research, Savoy Foundation and Canada Foundation for Innovation. We are part of the Neuroscience Axis of the CHU de Québec Research Center (CRCHUQ) in Québec-city.
Tyan L, Chamberland S, Magnin E, Camiré O, Francavilla R, David LS, Deisseroth K, Topolnik L
J Neurosci. 2014 Mar 26;34(13):4534-47. doi: 10.1523/JNEUROSCI.3813-13.2014.
Dendritic inhibition provided by interneuron-specific cells controls the firing rate and timing of the hippocampal feedback inhibitory circuitry.
We've moved to the CHUL Research Center of CRCHU de Québec. Located in the new Genomics Center and within the Intravital Imaging Facility, the lab is immersed in a vibrant, collaborative environment with a generous ongoing support.