2A) and fourfold (0.05; Fig. Soluble Factors that Enhance Liver Restoration by Reducing Fibrosis While Keeping Regeneration inside a Model of Chronic Liver Injury Ki67_A6_and_PANCK_IHC.tif (7.2M) GUID:?0DB94CC6-E3D8-4ADA-939E-D6EAF931D494 Supplemental Material, Ki67_A6_and_PANCK_IHC for Human being Amnion Epithelial Cells Produce Soluble Factors that Enhance Liver Restoration by Reducing Fibrosis While Maintaining Regeneration inside a Model of Chronic Liver Injury by Alexander Hodge, Neil Andrewartha, Dinushka Lourensz, Robyn Strauss, Jeanne Correia, Mihiri Goonetilleke, George Yeoh, Rebecca Lim and William Sievert in Cell Transplantation Supplemental Material, Supp_fig_4_cytokines – Human being Amnion Epithelial Cells Produce Soluble Factors that Enhance Liver Restoration by Reducing Fibrosis While Maintaining Regeneration inside a Model of Chronic Liver Injury Supp_fig_4_cytokines.jpg (64K) GUID:?66A60755-3895-492B-A286-3F9479027C02 Supplemental Material, Supp_fig_4_cytokines for Human being Amnion Epithelial Cells Produce Soluble Factors that Enhance Liver Repair by Reducing Fibrosis While Maintaining Regeneration inside a Model of Chronic Liver Injury by Alexander Hodge, Neil Andrewartha, Dinushka Lourensz, Robyn Strauss, Jeanne Correia, Mihiri Goonetilleke, George Yeoh, Rebecca Lim and William Sievert in Cell Transplantation Supplemental Material, Supp_Fig_5_FN14_and_GP130 – Human being Amnion Epithelial Cells Produce Soluble Factors that Enhance Liver Repair by Reducing Fibrosis While Maintaining Regeneration inside a Model of Chronic Liver Injury Supp_Fig_5_FN14_and_GP130.jpg (29K) GUID:?C045782D-922D-4123-BAEB-115E0733AAC0 Supplemental Material, Supp_Fig_5_FN14_and_GP130 for Human being Amnion Epithelial Cells Produce Soluble Factors that Enhance Liver Repair by Reducing Fibrosis While Maintaining Regeneration inside a Model of Chronic Liver Injury by Alexander Hodge, Neil Andrewartha, Dinushka Lourensz, Robyn Strauss, Jeanne Correia, Mihiri Goonetilleke, George Yeoh, Rebecca Lim and William Sievert in Cell Transplantation Supplemental Material, Supp_fig_6_Heatmap_of_markers – Human being Amnion Epithelial Cells Produce Soluble Factors that Enhance Liver Repair by Reducing Fibrosis While Maintaining Regeneration inside a Model of Chronic Liver Injury Supp_fig_6_Heatmap_of_markers.jpg (96K) GUID:?9588F62E-2A9D-44CE-BB91-4A0140A848BE Supplemental Material, Supp_fig_6_Heatmap_of_markers for Human being Amnion Epithelial SKI-II Cells Produce Soluble Factors that Enhance Liver Repair by Reducing Fibrosis While Maintaining Regeneration inside a Model of Chronic Liver Injury by Alexander Hodge, Neil Andrewartha, Dinushka Lourensz, Robyn Strauss, Jeanne Correia, Mihiri Goonetilleke, George Yeoh, Rebecca Lim and William Sievert in Cell Transplantation Supplemental Material, Treatment_outline – Human being Amnion Epithelial Cells Produce Soluble Factors that Enhance Liver Repair by Reducing Fibrosis While Maintaining Regeneration inside a Model of Chronic Liver Injury Treatment_outline.jpg (1.6M) GUID:?A7C07F9A-6510-4E24-ABBB-69CEE6EC6F13 Supplemental Material, Treatment_outline for Human being Amnion Epithelial Cells Produce Soluble Factors that Enhance Liver Repair by Reducing Fibrosis While Maintaining Regeneration inside a Model of Chronic Liver Injury by Alexander Hodge, Neil Andrewartha, Dinushka Lourensz, Robyn Strauss, Jeanne Correia, Mihiri Goonetilleke, George Yeoh, Rebecca Lim SKI-II and William Sievert in Cell Transplantation Abstract Human being amnion epithelial cells (hAECs) exert potent antifibrotic and anti-inflammatory effects when transplanted into preclinical models of tissue fibrosis. These effects are mediated in part via the secretion of soluble factors by hAECs which modulate signaling pathways and impact cell types involved in inflammation and fibrosis. Based on these reports, we hypothesized that these soluble factors may also support liver regeneration during chronic liver injury. To test this, we characterized the effect of both hAECs and hAEC-conditioned medium (CM) on SKI-II liver repair in a mouse model of carbon tetrachloride (CCl4)-induced fibrosis. Liver repair was assessed by liver fibrosis, hepatocyte proliferation, and the liver progenitor cell (LPC) response. We found that the Rabbit Polyclonal to CSFR administration of hAECs or hAEC-CM reduced liver injury and fibrosis, sustained hepatocyte proliferation, and reduced LPC figures during chronic liver injury. Additionally, we undertook in vitro studies to document both the cellCcell and paracrine-mediated effects.