in matrices of reconstituted Matrigel with increasing levels of fibronectin [22] and is also visible in our personal control experiments using collagen hydrogels with different amounts of fibronectinone of the main components of Matrigel-. M (b) and CM+, M+ (d). Snapshots display cell migration in the different hydrogels towards 20% FBS.(TIF) pone.0171417.s003.tif (3.4M) GUID:?418BE8D6-52D1-4017-9569-420C2DEEE639 S4 Fig: Quantification of H1299 cell migration capability in 3D Transwell experiments. Migration collapse, relative to M hydrogels of all the explained hydrogels, in the presence or absence of serum. The number of replicas of each experiment is definitely 8 for 20% FBS experiments and 4 for those LY2119620 without LY2119620 serum. *** shows very statistically significant difference of Anova One-Way analysis of variances followed by Bonferroni post-hoc test (p<0.005).(TIF) pone.0171417.s004.tif (175K) GUID:?03C57504-1217-45CD-B116-FAACFAC1A807 S5 Fig: Quantification of H1299 cell migration in hydrogels with increasing levels of cross-linking or fibronectin content. LY2119620 A: Serum free. B: 20%FBS. The number of cells analyzed was: TG-6: 176 (A), 178 (B); TG-13: 271 (A), 303 (B); TG-26 250 (A), 231 (B); TG-F10 162 (A), 211 (B); TG-F20 164 (A), 135 (B). *** shows very statistically significant difference of Anova One-Way analysis of variances followed by Bonferroni post-hoc test (p<0.005).(TIF) pone.0171417.s005.tif (78K) GUID:?AE0F3B4B-247C-4959-A8B3-30D6B73B02BC S6 Fig: Morphological characterization of the hydrogels used in the control experiments from confocal images. Average and standard deviation (std) of the morphological measurement from Confocal Reflection Microscopy images. The number of samples used to calculate the Dietary fiber size, Dietary fiber persistence, and Pore size is definitely nine (n = 3) since we analyzed three sub-images from each type. * Indicates statistically significant difference of non-parametric Mann-Whitney U-test (p<0.05).(TIF) pone.0171417.s006.tif (286K) GUID:?3FE1559A-D34E-43C8-8094-A3784403036F S1 Table: Morphological characterization of the hydrogels from SEM images. Average and standard deviation (std) of the morphological measurement from the SEM images. The number of samples used to calculate the %Porosity, Dietary fiber diameter and Quantity of pores and is three (n = 3) since we analyzed three images LY2119620 from each type. The number of samples used to calculate Pore size assorted between sample types, since the unit used was the pore. Namely, the n ideals were n = 1830 (C), n = 1012 (CM) and n = 487 (CM+).(DOCX) pone.0171417.s007.docx (39K) GUID:?2842884E-7965-4FCE-9392-0C5402F74930 S2 Table: Morphological characterization of the hydrogels from confocal images. Average and standard deviation (std) of the morphological measurement from the Confocal Reflection Microscopy images. The number of samples used to calculate the Dietary fiber length, Dietary fiber persistence, and Pore size is definitely nine (n = 9) since we analyzed nine sub-images from each type.(DOCX) pone.0171417.s008.docx (36K) GUID:?1CF4042C-682C-4F3F-8804-C991C5A14B03 S3 Table: Migration experiments. LY2119620 Mean and standard error (parenthesis) of accumulated range (in microns) after 12 hours of migration, and rate of migration (in microns per hour) in hydrogels C, CM and CM+, with no chemo-attracting compound (Control) using serum comprising medium, (20% FBS) or after conjugation with integrin-blocking antibodies (20% FBS + Anti-1, 20% FBS + Anti-3 and 20% FBS + Anti-1+3).(DOCX) pone.0171417.s009.docx (54K) GUID:?A86A1C02-EF43-401F-87B5-6D67DFDA099C S4 Table: Transwell invasion experiments. Mean and standard error (parenthesis) of quantity of invading cells in C, CM, and CM+, hydrogels, and in hydrogels with Matrigel only at equal concentration as with CM (M, 2mg/ml) and CM+ (M+, 4mg/ml). The number of replicas of each experiment is definitely 8 for 20%FBS, and 4 for serum free, Control experiments.(DOCX) pone.0171417.s010.docx (34K) GUID:?B5928747-2055-4603-B469-EAEB4AA27500 S5 Table: Mechanical characterization of the hydrogels. Average (avg) and standard deviation (std) of the storage (G) and loss (G) moduli, both in pascal devices (Pa) of the control, Matrigel only containing hydrogels. The standard deviation corresponds to three repetitions of each experiment (n = 3).(DOCX) pone.0171417.s011.docx (29K) GUID:?BEC10361-D51A-406E-AC54-0807CB862CBA S6 Table: Control experiments. Mean and standard error (parenthesis) of accumulated range (in microns) after 12 hours of migration in collagen only hydrogels with increasing crosslinking levels TG6, TG13, and TG26, and with increasing levels of fibronectin TG-F5, TG-F10 and TG-F20, with no chemo-attracting compound (Control) and using serum comprising medium, (20% FBS). The figures are average ideals obtain in two migration experiments.(DOCX) pone.0171417.s012.docx (38K) GUID:?79811E0A-3767-4700-8FC5-64209E89023F S7 Table: Morphological characterization of the hydrogels CUL1 used in the control experiments from confocal images. Average and standard deviation (std) of the morphological measurement from Confocal Reflection Microscopy images. The number of samples used to calculate the Dietary fiber.