These results indicated that miR\342\3p overexpression improved the radiosensitivity of osteosarcoma cells. Open in a separate window FIGURE 4 Effect of miR\342\3p overexpression on radiosensitivity of osteosarcoma cells. or miR\342\3p overexpression enhanced the radiosensitivity of osteosarcoma cells. Moreover, LINC00210 increased GFRA1 expression via sponging miR\342\3p. Additionally, LINC00210 knockdown improved the radiosensitivity of osteosarcoma cells by regulating GFRA1 expression via sponging miR\342\3p. Conclusion LINC00210 modulated the radiosensitivity of osteosarcoma cells via the miR\342\3p/GFRA1 axis, making LINC00210 a novel target for improving radiotherapy efficiency in osteosarcoma. and test, one\way ANOVA, and two\way ANOVA. Pearson’s correlation analysis decided the correlation. em P /em ? ?.05 was identified as statistically significant. 3.?RESULTS 3.1. LINC00210 and GFRA1 were up\regulated, and miR\342\3p was down\regulated in osteosarcoma tissue The expression levels of LINC00210, miR\342\3p, and GFRA1 mRNA in osteosarcoma tissue were determined by qRT\PCR assay. Results showed that LINC00210 was up\regulated in osteosarcoma tissue compared to adjacent healthy tissues ( em P /em ? ?.05, Figure?1A). Besides, miR\342\3p was down\regulated in osteosarcoma tissue compared Licofelone to adjacent healthy tissues ( em P /em ? ?.05, Figure?1B). The mRNA expression of GFRA1 was up\regulated in osteosarcoma tissue compared to adjacent healthy tissues ( em P /em ? ?.05, Figure?1C). In addition, Pearson’s correlation analysis results revealed a negative correlation between LINC00210 and miR\342\3p expression in osteosarcoma ( em P /em ? ?.05, Figure?1D). There was a positive correlation between LINC00210 and GFRA1 expression in osteosarcoma ( em P /em ? ?.05, Figure?1E), while a negative correlation between miR\342\3p and GFRA1 expression in osteosarcoma was Licofelone present ( em P /em ? ?.05, Figure?1F). Therefore, LINC00210 and GFRA1 were up\regulated, and miR\342\3p was down\regulated in osteosarcoma tissue. Open in a Licofelone separate window Physique 1 The expression levels of LINC00210, miR\342\3p, and GFRA1 in osteosarcoma tissue. A, The expression of LINC00210 in osteosarcoma tissues and adjacent healthy tissues was determined by qRT\PCR. B, The expression of miR\342\3p in osteosarcoma tissues and adjacent Licofelone healthy tissues was determined by qRT\PCR. C, The mRNA expression of GFRA1 in osteosarcoma tissues and adjacent healthy tissues was determined by qRT\PCR. D, The relationship between LINC00210 and miR\342\3p expression was analyzed by Pearson’s correlation analysis. E, The relationship between LINC00210 and GFRA1 expression was analyzed by Pearson’s correlation analysis. F, Pearson’s correlation analysis analyzed the relationship of miR\342\3p and GFRA1 expression. * em P /em ? ?.05 3.2. LINC00210 and GFRA1 were up\regulated, and miR\342\3p was down\regulated CDC7L1 in osteosarcoma cells The above results found that LINC00210 and GFRA1 were up\regulated, and miR\342\3p was down\regulated in osteosarcoma tissue. To further clarify the expression levels of LINC00210, miR\342\3p, and GFRA1 in osteosarcoma cells, we performed qRT\PCR assay. Results showed that this expression of LINC00210 was increased in osteosarcoma cell lines SOSP\9607, U\2OS, and F5M2 compared to human osteoblast cell line hFOB1.19 (all em P /em ? ?.05, Figure?2A). In addition, miR\342\3p was decreased in SOSP\9607, U\2OS, and F5M2 cells compared to hFOB1.19 cells (all em P /em ? ?.05, Figure?2B). The mRNA expression of GFRA1 was also higher in SOSP\9607, U\2OS, and F5M2 cells than that in hFOB1.19 cells ( em P /em ? ?.05, Figure?2C). Furthermore, the expression levels of LINC00210, miR\342\3p, and GFRA1 in osteosarcoma cells exposed to 4?Gy irradiation treatment were also determined. Results revealed that LINC00210 was up\regulated in osteosarcoma cells after irradiation treatment in a time\dependent manner ( em P /em ? ?.05, Figure?2D). However, the expression of miR\342\3p was decreased in osteosarcoma cells after irradiation treatment in a time\dependent manner ( em P /em ? ?.05, Figure?2E). Moreover, the expression level of GFRA1 was increased with the prolonged duration of irradiation treatment ( em P /em ? ?.05, Figure?2F). Hence, LINC00210 and GFRA1 Licofelone were up\regulated, and miR\342\3p was down\regulated in osteosarcoma cells with irradiation treatment. Open in a separate window Physique 2 The expression levels of LINC00210, miR\342\3p, and GFRA1 in osteosarcoma cells. A, The expression of LINC00210 in human osteoblast cell line hFOB1.19 and osteosarcoma cell lines SOSP\9607, U\2OS, and F5M2 was determined by qRT\PCR. B, The expression of miR\342\3p in human osteoblast cell line hFOB1.19 and osteosarcoma.