Compared with the model control group, the lesions of the treatment group significantly improved (Fig.?6c, ?,f,f, ?,i,i, ?,l,l, ?,o).o). group. Red fluorescence revealed immune complex deposition in the kidneys from your model group. Conclusions The combined intraperitoneal injection of pristane and LPS is the best way to induce SLE pathological changes. The pathological changes improved after UC-MSC treatment. Electronic supplementary material The online version of this article (doi:10.1186/s13287-016-0385-1) contains supplementary material, which is available to authorized users. Chinese tree shrews that had been domesticated from the Institute of Medical Biology, Chinese Academy of Medical Sciences in the Tree Shrew Germplasm Source Center were randomly divided into four groups of 20. The organizations received one of the following treatments: intraperitoneal injection of 1 1?ml pristane, intraperitoneal injection of 1 1?ml lipopolysaccharide (LPS), intraperitoneal injection with pristane and LPS, and no injection (normal settings). Pristane and LPS were purchased from Sigma Chemical Co.; LPS was dissolved to 0.5?mg/ml, and the injection volume was 1?ml per tree shrew. LPS and pristane were injected once every week for 3?weeks. After injection for 1, 2, or 3?weeks, the serum was collected and packaged in an ELISA plate. HRP-labeled rabbit anti-monkey IgG antibody was used to observe serum IgG changes. Each tree shrew serum sample was then sent to a medical laboratory to detect match C3 levels. Quantitative PCR Blood (0.5?ml) was collected from all tree shrews in each group. RNA was extracted using a blood RNA extraction kit from Baitaike according to the manufacturers instructions. Reverse transcription was carried out using the reverse transcription kit from Thermo according to the manufacturers instructions. Quantitative PCR was carried out using Thermo quantitative PCR reagents to detect the relative manifestation of IL-17 and Foxp3. The primer sequences and product lengths are offered IQ 3 in Table?1. The relative manifestation of IL-17 and Foxp3 was normalized by comparison with gene was more than twice that of the normal control group, as the comparative expression from the gene was significantly less than 0.5 that of the standard control group. Labeling and transplantation of tree shrew UC-MSCs Ten model tree shrews had been split into the model control group and the procedure group with five pets per group, and five normal tree shrews had IQ 3 been randomly chosen as the standard control group then. The UC-MSCs of tree shrews had been digested with 0.25?% trypsin, as well as the digestive function was terminated with full medium formulated with 20?% FBS. The cells had been pipetted uniformly, aspirated right into a 15?ml centrifuge pipe, and counted. The cells had been tagged at a focus of just one 1??106 cells/ml, and 1?ml of the cell suspension system was put into 5?l of the 3?mM stock options solution of DiR. The ensuing blend was incubated at 37?C for 10?mins and IQ 3 washed 3 x with prewarmed serum-free moderate (centrifugal rotation: 2000 rev/min, centrifugation period: 5?mins). The tagged cells (1??106 cells) were injected in to the tail blood vessels of treatment group and regular control group pets. ELISA recognition of serum antinuclear and antiphospholipid antibodies Fourteen days after cell transplantation, venous bloodstream was gathered from three sets of tree shrews. The serum was separated to detect antinuclear and antiphospholipid antibody changes. The antiphospholipid ELISA package was bought from Abcam Business as well as the Flt3 antinuclear antibody ELISA package was bought from ALPHA DIAGNOSTIC Business. The operating steps were followed according to kit instructions. Three sets of tree shrews: urinary proteins quantitation Fourteen days after cell transplantation, tree shrew morning hours urine was gathered from three groupings. The urinary proteins concentration was discovered with the Bradford technique. The proteins assay package was bought from Biyuntian Business. The steps had been followed in tight accordance using the package instructions. Three sets of tree shrews: serum inflammatory cytokine antibody microarray evaluation Fourteen days after cell transplantation, venous bloodstream was gathered from three sets of tree shrews. Serum was separated to detect inflammatory cytokine antibodies by microarray. The potato chips were bought from Raybiotech Business. The detection steps were followed according to.